Temporary

Part:BBa_K223025:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-07-06)


HlyR + HlyC + Hly B + Hly D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2891
    Illegal PstI site found at 2467
    Illegal PstI site found at 4119
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2891
    Illegal PstI site found at 2467
    Illegal PstI site found at 4119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2891
    Illegal BamHI site found at 1223
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2891
    Illegal PstI site found at 2467
    Illegal PstI site found at 4119
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2891
    Illegal PstI site found at 2467
    Illegal PstI site found at 4119
    Illegal AgeI site found at 2639
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183
    Illegal SapI.rc site found at 3737


Design Notes

HlyR + HlyC + Hly B + Hly D



Source

HlyR + HlyC + Hly B + Hly D


References